Crystallization and preliminary X-ray analysis of recombinant bovine cellular retinoic acid-binding protein.
1994 (English)In: Acta Crystallogr D Biol Crystallogr, ISSN 0907-4449, Vol. 50, no Pt 4, 370-4 p.Article in journal (Refereed) Published
Crystals of bovine cellular retinoic acid-binding protein (CRABPI) have been grown from protein expressed in E. coli. Two different crystal forms were obtained. Crystals containing protein with the ligand all-trans retinoic acid belong to space group P4(1) or P4(3), a = b = 41.36, c = 202.71 A and diffract to 2.5 A. Crystals of CRABP with the synthetic retinoid analogue Am80 diffract to 1.9 A with space group P2(1) and cell dimensions a = 37.03, b = 105.93, c = 40.31 A, beta = 110.28 degrees. Considerations in the crystallization of proteins with light-sensitive ligands are discussed.
Place, publisher, year, edition, pages
1994. Vol. 50, no Pt 4, 370-4 p.
IdentifiersURN: urn:nbn:se:uu:diva-21225PubMedID: 15299387OAI: oai:DiVA.org:uu-21225DiVA: diva2:48998