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Protein profiling of genomic instability in endometrial cancer
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles va¨g 2, 17177 Stockholm, Sweden.
Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Scheeles va¨g 2, 17177 Stockholm, Sweden.
Department of Surgery, University of Lu¨beck, 23538 Lu¨beck, Germany.
Institute of Medical Biometry and Statistics, University of Lu¨beck, 23562 Lu¨beck, Germany.
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2012 (English)In: Cellular and Molecular Life Sciences (CMLS), ISSN 1420-682X, E-ISSN 1420-9071, Vol. 69, no 2, 325-333 p.Article in journal (Refereed) Published
Abstract [en]

DNA aneuploidy has been identified as a prognostic factor in the majority of epithelial malignancies. We aimed at identifying ploidy-associated protein expression in endometrial cancer of different prognostic subgroups. Comparison of gel electrophoresis-based protein expression patterns between normal endometrium (n = 5), diploid (n = 7), and aneuploid (n = 7) endometrial carcinoma detected 121 ploidy-associated protein forms, 42 differentially expressed between normal endometrium and diploid endometrioid carcinomas, 37 between diploid and aneuploid endometrioid carcinomas, and 41 between diploid endometrioid and aneuploid uterine papillary serous cancer. Proteins were identified by mass spectrometry and evaluated by Ingenuity Pathway Analysis. Targets were confirmed by liquid chromatography/mass spectrometry. Mass spectrometry identified 41 distinct polypeptides and pathway analysis resulted in high-ranked networks with vimentin and Nf-kappa B as central nodes. These results identify ploidy-associated protein expression differences that overrule histopathology-associated expression differences and emphasize particular protein networks in genomic stability of endometrial cancer.

Place, publisher, year, edition, pages
Springer , 2012. Vol. 69, no 2, 325-333 p.
Keyword [en]
Aneuploidy, Endometrial carcinoma, Genomic instability, Mass spectrometry, Pathway analysis, Two-dimensional gel electrophoresis
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-168100DOI: 10.1007/s00018-011-0752-0ISI: 000298653500011OAI: oai:DiVA.org:uu-168100DiVA: diva2:492112
Available from: 2012-02-07 Created: 2012-02-06 Last updated: 2017-12-08Bibliographically approved

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Hellman, Ulf

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