Approaches for increasing throughput andinformation content of image-based zebrafishscreens
2011 (English)In: Proceeding of SSBA 2011, 2011Conference paper (Other academic)
Microscopy in combination with image analysis has emerged as one of the most powerful and informativeways to analyze cell-based high-throughput screening (HTS) samples in experiments designed to uncover novel drugs and drug targets. However, many diseases and biological pathways can be better studied in whole animals, particularly diseases and pathways that involve organ systems and multicellular interactions, such as organ development, neuronal degeneration and regeneration, cancer metastasis, infectious disease progression and pathogenesis. The zebrafish is a wide-spread and popular vertebrate model of human organfunction and development, and it is unique in the sense that large-scale in vivo genetic and chemical studies are feasible due in part to its small size, optical transparency,and aquatic habitat. To improve the throughput and complexity of zebrafish screens, a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae has been developed at Yanik lab at Research Laboratory of Electronics, MIT, USA. The system loads live zebrafish from reservoirs or multiwell plates, positions and rotates them for high-speed confocal imaging of organs,and dispenses the animals without damage. We present two improvements to the described system, including automation of positioning of the animals and a novel approach for brightfield microscopy tomographic imaging of living animals.
Place, publisher, year, edition, pages
Medical Image Processing Other Computer and Information Science
Research subject Computerized Image Processing; Computerized Image Analysis
IdentifiersURN: urn:nbn:se:uu:diva-168378OAI: oai:DiVA.org:uu-168378DiVA: diva2:495990
SSBA Symposium 2011