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Giardia lamblia-induced changes in gene expression in differentiated Caco-2
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology. Mikrobiologi. (Svärd)
2005 (English)In: Infect Immun, ISSN 0019-9567, Vol. 73, no 12, 8204-8 p.Article in journal (Other scientific) Published
Place, publisher, year, edition, pages
2005. Vol. 73, no 12, 8204-8 p.
Keyword [en]
Animals, Caco-2 Cells, Cell Differentiation, Chemokines/*genetics, Chemokines; CC/genetics, Epithelial Cells/cytology/parasitology, Gene Expression Regulation, Genome; Protozoan/genetics, Giardia lamblia/*pathogenicity, Humans, Intestinal Mucosa/cytology/*parasitology, Macrophage Inflammatory Proteins/genetics, Oligonucleotide Array Sequence Analysis
National Category
Microbiology in the medical area
URN: urn:nbn:se:uu:diva-22360PubMedID: 16299316OAI: oai:DiVA.org:uu-22360DiVA: diva2:50133
Available from: 2007-01-16 Created: 2007-01-16 Last updated: 2011-01-12
In thesis
1. Host-Pathogen Responses during Giardia infections
Open this publication in new window or tab >>Host-Pathogen Responses during Giardia infections
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Giardia lamblia is a eukaryotic parasite of the upper small intestine of humans and animals. The infecting trophozoite cells do not invade the epithelium lining of the intestine, but attach to the brush border surface in the intestinal lumen. The giardiasis disease in humans is highly variable. Prior to this study, the molecular mechanisms involved in establishment of infection or cause of disease were largely uncharacterized.

In this thesis, the molecular relationship between Giardia and the human host is described. The interaction of the parasite with human epithelial cells was investigated in vitro. Changes in the transcriptome and proteome of the parasite and the host cells, and changes in the micro-environment of the infection have been identified using microarray technology, and 1- and 2-Dimensional SDS-PAGE protein mapping together with mass spectrometry identification.

The first large-scale description of cellular activities within host epithelial cells during Giardia infection is included in this thesis (Paper I). We identified a unique activation of the host immune response and induction of apoptosis upon infection by Giardia. Four important virulence factors of the parasite, directly linked to the success of Giardia infection, were characterized and are presented in Papers II and III. The parasite was shown to have immune-modulating capacities, and to release proteins during host-interaction that facilitate the establishment of infection. Additional putative virulence factors were found among Giardia genes transcriptionally up-regulated during early infection (Paper IV).

In summary, this thesis provides important insights into the molecular mechanisms of the host-parasite interaction.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2009. 71 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 689
Giardia, parasite infection, host-parasite releationship, immune activation, virulence factor, immune evasion
National Category
Biochemistry and Molecular Biology Microbiology
Research subject
urn:nbn:se:uu:diva-108980 (URN)978-91-554-7652-6 (ISBN)
Public defence
2009-12-12, B41, Biomedicinskt Centrum, Husargatan 3, Uppsala, 10:00 (English)
Available from: 2009-11-19 Created: 2009-10-06 Last updated: 2009-11-19Bibliographically approved

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