Particle enhanced turbidimetric immunoassay for the determination of urine cystatin C on Cobas c501
2012 (English)In: Clinical Biochemistry, ISSN 0009-9120, E-ISSN 1873-2933, Vol. 45, no 4-5, 339-344 p.Article in journal (Refereed) Published
Urinary cystatin C has been reported to be a good marker for tubular damage and acute kidney injury. The aim of this study was to develop a high throughput assay for the quantification of urine cystatin C.
Antigen-excess, imprecision, interference, linearity, recovery, sample stability and reference values were evaluated on Cobas c501.
The assay was linear over the dynamic range of the study (R(2)=0.9994). The total assay imprecision was below 5%. The assay recovery was estimated at 87-100%. No tendency to antigen-excess (up to 35mg/L), nor interference with haemoglobin (1.25-10g/L) was observed. Cystatin C was stable for 1day at ambient temperature (19-23°C) but for 2days at +4°C. The reference interval for cystatin C in urine was <0.414mg/L.
The urinary cystatin C assay verified to be a reliable assay with convenient performance characteristics, enabling routine testing on clinical chemistry platforms.
Place, publisher, year, edition, pages
2012. Vol. 45, no 4-5, 339-344 p.
Clinical Laboratory Medicine
IdentifiersURN: urn:nbn:se:uu:diva-168693DOI: 10.1016/j.clinbiochem.2011.12.027ISI: 000302844500013PubMedID: 22266397OAI: oai:DiVA.org:uu-168693DiVA: diva2:503187