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Particle enhanced turbidimetric immunoassay for the determination of urine cystatin C on Cobas c501
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Chemistry.
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2012 (English)In: Clinical Biochemistry, ISSN 0009-9120, E-ISSN 1873-2933, Vol. 45, no 4-5, 339-344 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVE:

Urinary cystatin C has been reported to be a good marker for tubular damage and acute kidney injury. The aim of this study was to develop a high throughput assay for the quantification of urine cystatin C.

METHODS:

Antigen-excess, imprecision, interference, linearity, recovery, sample stability and reference values were evaluated on Cobas c501.

RESULTS:

The assay was linear over the dynamic range of the study (R(2)=0.9994). The total assay imprecision was below 5%. The assay recovery was estimated at 87-100%. No tendency to antigen-excess (up to 35mg/L), nor interference with haemoglobin (1.25-10g/L) was observed. Cystatin C was stable for 1day at ambient temperature (19-23°C) but for 2days at +4°C. The reference interval for cystatin C in urine was <0.414mg/L.

CONCLUSIONS:

The urinary cystatin C assay verified to be a reliable assay with convenient performance characteristics, enabling routine testing on clinical chemistry platforms.

Place, publisher, year, edition, pages
2012. Vol. 45, no 4-5, 339-344 p.
National Category
Clinical Laboratory Medicine
Identifiers
URN: urn:nbn:se:uu:diva-168693DOI: 10.1016/j.clinbiochem.2011.12.027ISI: 000302844500013PubMedID: 22266397OAI: oai:DiVA.org:uu-168693DiVA: diva2:503187
Available from: 2012-02-15 Created: 2012-02-15 Last updated: 2017-12-07Bibliographically approved

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Sohrabian, AzitaLarsson, Anders

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