uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
DNA binding activity of Helicobacter pylori DnaB helicase: the role of the N-terminal domain in modulating DNA binding activities
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Microbiology.
Show others and affiliations
2012 (English)In: The FEBS Journal, ISSN 1742-464X, E-ISSN 1742-4658, Vol. 279, no 2, 234-250 p.Article in journal (Refereed) Published
Abstract [en]

Replicative helicases are major motor proteins essential for chromosomal DNA replication in prokaryotes. Usually hexameric in solution, their DNA binding property must have different roles at stages ranging from the loading onto a branched structure at initiation from the origin to the highly processive translocation during elongation. Here, we have analysed the DNA binding activity of Helicobacter pylori (Hp) replicative helicase, DnaB. The results indicate that while the C-terminal region is important for its DNA binding activity, the N-terminus appears to dampen the proteins affinity for DNA. The masking activity of the N-terminus does not require ATP or hexamerization of HpDnaB and can be overcome by deleting the N-terminus. It can also be neutralized by engaging the N-terminus in an interaction with a partner like the C-terminus of DnaG primase. The inhibitory effect of the N-terminus on DNA binding activity is consistent with the 3D homology model of HpDnaB. Electron microscopy of the HpDnaBssDNA complex showed that HpDnaB preferentially bound at the ends of linear ssDNA and translocated along the DNA in the presence of ATP. These results provide an insight into the stimulatory and inhibitory effects of different regions of HpDnaB on DNA binding activities that may be central to the loading and translocation functions of DnaB helicases.

Place, publisher, year, edition, pages
2012. Vol. 279, no 2, 234-250 p.
Keyword [en]
DNA binding, DNA replication, DnaB helicase, Helicobacter pylori, ssDNA
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-168740DOI: 10.1111/j.1742-4658.2011.08418.xISI: 000298746000005OAI: oai:DiVA.org:uu-168740DiVA: diva2:503712
Available from: 2012-02-16 Created: 2012-02-15 Last updated: 2012-02-16Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Dasgupta, Santanu
By organisation
In the same journal
The FEBS Journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 164 hits
ReferencesLink to record
Permanent link

Direct link