uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Expression of heparanase in vascular cells and astrocytes of the mouse brain after focal cerebral ischemia
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Public Health and Caring Sciences, Geriatrics.
Show others and affiliations
2012 (English)In: Brain Research, ISSN 0006-8993, E-ISSN 1872-6240, Vol. 1433, 137-144 p.Article in journal (Refereed) Published
Abstract [en]

Heparanase is a heparan sulfate degrading endoglycosidase. Previous work has demonstrated that heparanase plays important roles in various biological processes including angiogenesis, wound healing and metastasis. However, the role of heparanase in the post-ischemic brain is not well defined. Transient focal cerebral ischemia in adult mice was induced by ligations of the right middle cerebral artery (MCA) and both common carotid arteries (CCAs). All mice were subjected to bromodeoxyuridine (BrdU) injection and sacrificed at different time points after stroke for immunohistochemical and Western blot analyses. Heparanase expression increased after ischemia in both cell-specific and time-dependent manners. Three to 7 days after stroke, levels of the 50-kD heparanase, basic fibroblast growth factor (FGF-2), and angiopoietin-2 (Ang-2) increased in the peri-infarct region. At early time points, heparanase expression was largely confined to proliferating vascular endothelial cells. At 14 days after ischemia, this expression had shifted to astrocytes in the same region. These data show that cerebral ischemia markedly increases heparanase levels in endothelial cells and then in astrocytes. The unique features of the heparanase upregulation imply that heparanase may play specific roles in the pathological and regenerative processes during the acute and sub-acute/chronic phases in the post-stroke brain.

Place, publisher, year, edition, pages
2012. Vol. 1433, 137-144 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-169158DOI: 10.1016/j.brainres.2011.11.032ISI: 000300534100016PubMedID: 22169133OAI: oai:DiVA.org:uu-169158DiVA: diva2:505201
Available from: 2012-02-23 Created: 2012-02-23 Last updated: 2012-03-19Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Li, Jin-PingZhang, Xiao
By organisation
Department of Medical Biochemistry and MicrobiologyGeriatrics
In the same journal
Brain Research
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 271 hits
ReferencesLink to record
Permanent link

Direct link