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High-capacity conductive nanocellulose paper sheets for electrochemically controlled extraction of DNA oligomers.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanoteknologi och funktionella material)ORCID iD: 0000-0002-5496-9664
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. (Nanoteknologi och funktionella material)
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2011 (English)In: PloS one, ISSN 1932-6203, Vol. 6, no 12, e29243- p.Article in journal (Refereed) Published
Abstract [en]

Highly porous polypyrrole (PPy)-nanocellulose paper sheets have been evaluated as inexpensive and disposable electrochemically controlled three-dimensional solid phase extraction materials. The composites, which had a total anion exchange capacity of about 1.1 mol kg(-1), were used for extraction and subsequent release of negatively charged fluorophore tagged DNA oligomers via galvanostatic oxidation and reduction of a 30-50 nm conformal PPy layer on the cellulose substrate. The ion exchange capacity, which was, at least, two orders of magnitude higher than those previously reached in electrochemically controlled extraction, originated from the high surface area (i.e. 80 m(2) g(-1)) of the porous composites and the thin PPy layer which ensured excellent access to the ion exchange material. This enabled the extractions to be carried out faster and with better control of the PPy charge than with previously employed approaches. Experiments in equimolar mixtures of (dT)(6), (dT)(20), and (dT)(40) DNA oligomers showed that all oligomers could be extracted, and that the smallest oligomer was preferentially released with an efficiency of up to 40% during the reduction of the PPy layer. These results indicate that the present material is very promising for the development of inexpensive and efficient electrochemically controlled ion-exchange membranes for batch-wise extraction of biomolecules.

Place, publisher, year, edition, pages
2011. Vol. 6, no 12, e29243- p.
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
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URN: urn:nbn:se:uu:diva-169219DOI: 10.1371/journal.pone.0029243PubMedID: 22195031OAI: oai:DiVA.org:uu-169219DiVA: diva2:505597
Available from: 2012-02-24 Created: 2012-02-24 Last updated: 2016-11-30Bibliographically approved

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Razaq, AamirNyström, GustavStrømme, MariaMihranyan, Albert

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