The in vitro modification of phosphorylated pyruvate kinase by a Ca2+-activated protease from rat liver
1980 (English)In: Acta Chemica Scandinavica, ISSN 0904-213X, Vol. 34, no 6, 419-422 p.Article in journal (Refereed) Published
A Ca/+-activated protease from rat liver cell sap was prepared. It was shown to act on rat liver pyruvate kinase that had been phosphorylated by the catalytic subunit of cyclic AMP-dependent protein kinase, the activity being optimum at neutral pH. The modified pyruvate kinase had the same Vmax as the phosphoenzyme but showed a lower affinity for the substrate phosphoenolpyruvate. The possibility that this proteolytic attack is the step that initiates further degradation in the cell is discussed.
Place, publisher, year, edition, pages
1980. Vol. 34, no 6, 419-422 p.
Other Medical Sciences not elsewhere specified
IdentifiersURN: urn:nbn:se:uu:diva-169297PubMedID: 7008473OAI: oai:DiVA.org:uu-169297DiVA: diva2:506090