Expression of perilipins in human skeletal muscle in vitro and in vivo in relation to diet, exercise and energy balance
2012 (English)In: Archives of Physiology and Biochemistry, ISSN 1381-3455, E-ISSN 1744-4160, Vol. 118, no 1, 22-30 p.Article in journal (Refereed) Published
The perilipin proteins enclose intracellular lipid droplets. We describe the mRNA expression of the five perilipins in human skeletal muscle in relation to fatty acid supply, exercise and energy balance. We observed that all perilipins were expressed in skeletal muscle biopsies with the highest mRNA levels of perilipin 2, 4 and 5. Cultured myotubes predominantly expressed perilipin 2 and 3. In vitro, incubation of myotubes with fatty acids enhanced mRNA expression of perilipin 1, 2 and 4. In vivo, low fat diet increased mRNA levels of perilipin 3 and 4. Endurance training, but not strength training, enhanced the expression of perilipin 2 and 3. Perilipin 1 mRNA correlated positively with body fat mass, whereas none of the perilipins were associated with insulin sensitivity. In conclusion, all perilipins mRNAs were expressed in human skeletal muscle. Diet as well as endurance exercise modulated the expression of perilipins.
Place, publisher, year, edition, pages
2012. Vol. 118, no 1, 22-30 p.
PAT-proteins, dietary fat, strength training, endurance training
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-169355DOI: 10.3109/13813455.2011.630009ISI: 000299743500005OAI: oai:DiVA.org:uu-169355DiVA: diva2:506846