In vivo protein crystallization opens new routes in structural biology
2012 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 9, no 3, 259-262 p.Article in journal (Refereed) Published
Protein crystallization in cells has been observed several times in nature. However, owing to their small size these crystals have not yet been used for X-ray crystallographic analysis. We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
Place, publisher, year, edition, pages
2012. Vol. 9, no 3, 259-262 p.
Structural Biology Biophysics
IdentifiersURN: urn:nbn:se:uu:diva-169657DOI: 10.1038/nmeth.1859ISI: 000300890400020OAI: oai:DiVA.org:uu-169657DiVA: diva2:507498