Low Charge Density Cationic Polymers for Gene Delivery: Exploring the Influence of Structural Elements on In Vitro Transfection
2012 (English)In: Macromolecular Bioscience, ISSN 1616-5187, E-ISSN 1616-5195, Vol. 12, no 6, 840-848 p.Article in journal (Refereed) Published
A series of end-functionalized poly(trimethylene carbonate) DNA carriers, characterized by low cationic charge density and pronounced hydrophobicity, was used to study structural effects on in vitro gene delivery. As the DNA-binding moieties were identical in all polymer structures, the differences observed between the different polymers were directly related to the functionality and length of the polymer backbone. The transfection efficiency and cytotoxicity of the polymer/DNA complexes were thus found to be dependent on a combination of polymer charge density and functionality, highlighting the importance of such structural considerations in the development of materials for efficient gene delivery.
Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2012. Vol. 12, no 6, 840-848 p.
amphiphiles, biodegradable, biological applications of polymers, gene delivery, transfection
Polymer Chemistry Biochemistry and Molecular Biology
Research subject Chemistry with specialization in Polymer Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-169673DOI: 10.1002/mabi.201100480ISI: 000304711200014OAI: oai:DiVA.org:uu-169673DiVA: diva2:507536