uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Long-Term impact of fertilization on activity and composition of bacterial communities and metabolic guilds in agricultural soil
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Evolution, Limnology. (Mikrobiell Ekologi)
Show others and affiliations
2007 (English)In: Soil Biology and Biochemistry, ISSN 0038-0717, E-ISSN 1879-3428, Vol. 39, no 1, 106-115 p.Article in journal (Refereed) Published
Abstract [en]

To explore long-term impact of organic and inorganic fertilizers on microbial communities, we targeted both the total bacterial community and the autotrophic ammonia oxidizing bacteria (AOB) in soil from six treatments at an experimental field site established in 1956: cattle manure, sewage sludge, Ca(NO3)2, (NH4)2SO4, unfertilized and unfertilized without crops. All plots, except the bare fallows, were cropped with maize. Effects on activity were assessed by measuring the basal respiration and substrate induced respiration (SIR) rates, and the potential activity of the AOB. To determine the bacterial community composition, 16S rRNA genes were used to fingerprint total soil communities by terminal restriction fragment length polymorphism analysis and AOB communities by denaturing gradient gel electrophoresis. The fertilization regimes had clear effects on both activity and composition of the soil communities. Basal respiration and r, which was kinetically derived as the exponentially growing fraction of the SIR-response, correlated well with the soil organic C content (r=0.93 and 0.66, respectively). Soil pH ranged from 3.97 to 6.26 in the treatments and was found to be an important factor influencing all microbial activities. pH correlated negatively with the ratio between basal respiration and SIR (r=0.90), indicating a decreased efficiency of heterotrophic microorganisms to convert organic carbon into microbial biomass in the most acid soils with pH 3.97 and 4.68 ((NH4)2SO4 and sewage sludge fertilized plots, respectively). The lowest SIR and ammonia oxidation rates were also found in these treatments. In addition, these treatments exhibited individually different community fingerprints, showing that pH affected the composition of AOB and total bacterial communities. The manure fertilized plots harbored the most diverse AOB community and the pattern was linked to a high potential ammonia oxidation activity. Thus, the AOB community composition appeared to be more strongly linked to the activity than the total bacterial communities were, likely explained by physiological differences in the populations present.

Place, publisher, year, edition, pages
2007. Vol. 39, no 1, 106-115 p.
Keyword [en]
Ammonia oxidizing bacteria, Bacterial communities, DGGE, Fertilization, Manure, Sewage sludge, Soil respiration, T-RFLP
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-22993DOI: 10.1016/j.soilbio.2006.06.015ISI: 000242720300010OAI: oai:DiVA.org:uu-22993DiVA: diva2:50766
Available from: 2008-01-28 Created: 2008-01-28 Last updated: 2011-04-08Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Bertilsson, Stefan
By organisation
In the same journal
Soil Biology and Biochemistry
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 208 hits
ReferencesLink to record
Permanent link

Direct link