uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Lateral Flow Immunoassay Using Europium (III) Chelate Microparticles and Time-Resolved Fluorescence for Eosinophils and Neutrophils in Whole Blood
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Surface Biotechnology.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Physical and Analytical Chemistry, Surface Biotechnology, Centre for Surface Biotechnology.
Show others and affiliations
2007 (English)In: Clinical Chemistry, ISSN 0009-9147, E-ISSN 1530-8561, Vol. 53, no 2, 342-348 p.Article in journal (Refereed) Published
Abstract [en]

Background: A simple point-of-care method for measuring leukocyte counts in a doctor’s office or emergency room could be of great importance. We developed a protocol for measuring cell count by disrupting the cell membrane and analyzing specific proteins within the cells and used it to analyze proteins from eosinophils and neutrophils.

Methods: Lateral immunochromatographic (ICR) assays have been developed for eosinophil protein X (EPX) and human neutrophil lipocalin (HNL) as measures of the concentration of eosinophils and neutrophils. The correlation between the lateral ICR assays and cell counting of eosinophils and neutrophils was performed manually and with an automated cell counter. RIA assays measuring the same analytes were also compared with the results from cell counting and lateral ICR assays.

Results: The optimized assays showed analytical detection limits below the clinical ranges of 3.36 µg/L and 2.05 µg/L for EPX and HNL, respectively. The recovery was 114.8%–122.8% for EPX and 94.5%–96.9% for HNL. The imprecision was 3%–17% CV for EPX over the whole range and 5%–16% CV for HNL. The correlation coefficients between manually counted cells and lateral ICR assays were 0.9 and 0.83 for EPX and HNL, respectively.

Conclusion: The numbers of eosinophils and neutrophils in small amounts of blood can be estimated in the point-of-care setting by means of fast lateral ICR assays of EPX and HNL.

Place, publisher, year, edition, pages
2007. Vol. 53, no 2, 342-348 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-22996DOI: 10.1373/clinchem.2006.074021ISI: 000243965500025PubMedID: 17185370OAI: oai:DiVA.org:uu-22996DiVA: diva2:50769
Available from: 2007-02-12 Created: 2007-02-12 Last updated: 2011-02-15Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Rundström, GerdVenge, Per
By organisation
Surface BiotechnologyCentre for Surface BiotechnologyClinical Chemistry
In the same journal
Clinical Chemistry
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 178 hits
ReferencesLink to record
Permanent link

Direct link