Sensitive detection of genes by sandwich hybridization and time-resolved fluorometry
1987 (English)In: Molecular and Cellular Probes, ISSN 0890-8508, E-ISSN 1096-1194, Vol. 1, no 2, 159-168 p.Article in journal (Refereed) Published
Europium has been used as a non-radioactive marker in immunoassays as this metal can be detected with high sensitivity by time-resolved fluorometry. In this work streptavidin labeled with europium was used to detect biotinylated probes in a sandwich nucleic-acid hybridization assay with microtitration strips as the solid phase. pBR 322 plasmids were detected with a sensitivity of 4 × 105 molecules. As the sample is added in solution in sandwich hybridization, fast and simple sample pre-treatment can be used without encountering background problems. The method was applied to test bacterial samples of uropathogenic Escherichia coli strains for the presence of the β-lactamase gene.
Place, publisher, year, edition, pages
1987. Vol. 1, no 2, 159-168 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-169911DOI: 10.1016/0890-8508(87)90024-7PubMedID: 3331173OAI: oai:DiVA.org:uu-169911DiVA: diva2:508017