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Isolation and immunologic properties of a heterogeneous antigen with the characteristics of the heavy chain of human plasma kininogen
1982 (English)In: Molecular Immunology, ISSN 0161-5890, E-ISSN 1872-9142, Vol. 19, no 1, 179-189 p.Article in journal (Refereed) Published
Abstract [en]

Kininogen antigen was purified from human plasma fraction IV by ion exchange chromatography, gel filtration and affinity chromatography with antibody specific immunoadsorbents. The immunologically pure glycoprotein had a mol. wt of approximately 60,000 and only one polypeptide chain by SDS-PAGE. An extensive charge heterogeneity by isoelectric focusing and gel filtration on polyacrylamide agarose could only in part depend on a comparatively high sialic acid content, but may be caused by differences in the carbohydrate structures sustained by lectin-binding heterogeneity on Con A-Sepharose. This antigen shares a dominating determinant with native plasma kininogens shown by complete patterns of identity in immunochemical analyses and with the monospecific antisera developed in rabbits against the heterogeneous components. The similar size, amino acid composition, low histidine content, lack of N-terminal amino acid and antigenic homogeneity fit all the so far known characteristics of the human kininogen heavy chain. Notably the antigenic determinant is resistant to degradation by activated kallikrein. This antigen with unimpaired immunologic activity may be a useful tool for preparation of antiserum for immunochemical determination of human plasma kininogen.

Place, publisher, year, edition, pages
1982. Vol. 19, no 1, 179-189 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-169927DOI: 10.1016/0161-5890(82)90260-7PubMedID: 7043244OAI: oai:DiVA.org:uu-169927DiVA: diva2:508057
Available from: 2012-03-07 Created: 2012-03-07 Last updated: 2012-03-30Bibliographically approved

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Syvänen, Ann-Christine
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