uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
A simple and reliable technique to combine oligonucleotide probe in situ hybridization with neuronal tract tracing in vertebrate embryos.
1996 (English)In: Biotechnic & Histochemistry, ISSN 1052-0295, E-ISSN 1473-7760, Vol. 71, no 6, 289-94 p.Article in journal (Refereed) Published
Abstract [en]

We describe a simple and reliable combination of in situ hybridization with neuronal tracing. The technique uses recent advances in the field of neuronal tract tracing including fast diffusing, low molecular weight dextran amines and fade resistant fluorescent dyes, and combines them with in situ hybridization using a sensitive oligonucleotide probe. Using this technique we have investigated the mRNA encoding the trkB receptor for brain-derived neurotrophic factor in identified facial and vestibular afferent and efferent neurons. We found very low levels of trkB mRNA in facial efferent neurons, whereas in the vestibular afferent neurons, clear labeling for the trkB mRNA could be seen. This technique can be applied to the developing embryo to study topology of a variety of cellular markers with reference to neuronal population or fibers identified by their origin or target.

Place, publisher, year, edition, pages
1996. Vol. 71, no 6, 289-94 p.
National Category
URN: urn:nbn:se:uu:diva-170015PubMedID: 8957555OAI: oai:DiVA.org:uu-170015DiVA: diva2:508177
Available from: 2012-03-07 Created: 2012-03-07 Last updated: 2012-03-07

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Hallböök, F
In the same journal
Biotechnic & Histochemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 246 hits
ReferencesLink to record
Permanent link

Direct link