Novel Cysteine Tags for the Sequencing of Non-Tryptic Disulfide Peptides of Anurans: ESI-MS Study of Fragmentation Efficiency
2011 (English)In: Journal of the American Society for Mass Spectrometry, ISSN 1044-0305, E-ISSN 1879-1123, Vol. 22, no 12, 2246-2255 p.Article in journal (Refereed) Published
Mass spectrometry faces considerable difficulties in de novo sequencing of long non-tryptic peptides with S-S bonds. Long disulfide-containing peptides brevinins 1E and 2Ec from frog Rana ridibunda were reduced and alkylated with nine novel and three known derivatizing agents. Eight of the novel reagents are maleimide derivatives. Modified samples were subjected to MS/MS studies on FT-ICR and Orbitrap mass spectrometers using CAD/HCD or ECD/ETD techniques. Procedures, fragmentation patterns, and sequence coverage for two peptides modified with 12 tags are described. ECD/ETD and CAD fragmentation revealed complementary sequence information. Higher-energy collisionally activated dissociation (HCD) sufficiently enhanced y-ions formation for brevinin 1E, but not for brevinin 2Ec. Some novel tags [N-benzylmaleimide, N-(2,6-dimethylphenyl)maleimide] along with known N-phenylmaleimide and iodoacetic acid showed high total sequence coverage taking into account combined ETD and HCD fragmentation. Moreover, modification of long (34 residues) brevinin 2Ec with N-benzylmaleimide or N-(2,6-dimethylphenyl) maleimide yielded high sequence coverage and full C-terminal sequence determination with ECD alone.
Place, publisher, year, edition, pages
2011. Vol. 22, no 12, 2246-2255 p.
Disulfide peptide, Brevinin, De novo sequencing, Cysteine tag, Maleimide, Electrospray ionization, ECD, ETD, CAD, HCD, FTICR-MS, Orbitrap
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-169984DOI: 10.1007/s13361-011-0247-0ISI: 000300360400016OAI: oai:DiVA.org:uu-169984DiVA: diva2:508327