Indole-related compounds from the National Cancer Institute (USA) repository do not inhibit HIV-1 replication in primary blood mononuclear cells
(English)Manuscript (preprint) (Other academic)
Indole derived substances have been shown previously to inhibit HIV-1 replication, presumably by interfering with cellular splicing factors of the SR protein family. Here we have tested the effect of indole-related substances obtained from the NCI repository on HIV-1 gene expression in transient transfections and in primary cell cultures infected by X4 and R5 tropic HIV-1 strains. Although these substances displayed a significant inhibitory effect on HIV-1 gene expression in transiently transfected cells, they did not inhibit replication of HIV-1 in primary peripheral blood mononuclear cells. These results demonstrate important differences between transfection assays performed in cell lines and infection assays performed in primary cells and indicate that only a subset of indole-related compounds can inhibit HIV-1 replication.
Research subject Medical Virology
IdentifiersURN: urn:nbn:se:uu:diva-170300OAI: oai:DiVA.org:uu-170300DiVA: diva2:508945