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Assessment of mucus thickness and production in situ
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrative Physiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology, Integrative Physiology.
2012 (English)In: Methods in molecular biology, ISSN 1064-3745, Vol. 842, 217-227 p.Article in journal (Refereed) Published
Abstract [en]

The nature of the mucus gel layer covering the gastrointestinal tract makes it difficult to study outside its natural site attached to the mucosa. Here, we describe a technique for intravital microscopy studies of the mucus gel layer from the stomach down to the colon in anesthetized rats and mice. Mucus thickness and accumulation rate in each segment of the gastrointestinal tract is measured with a micropipette technique under observation through a stereomicroscope. In this way, the nature of the mucus gel in vivo is readily studied, and effects of interventions or disease on the mucus can be determined in longitudinal studies or by comparing animals. Using this technique, we have been able to demonstrate that there are two forms of mucus gel adherent to the stomach and colon mucosa: one layer which is removable by suction and an underlying firm adherent gel layer, while in the small intestine, all mucus adhering to the mucosa can easily be removed.

Place, publisher, year, edition, pages
Springer Science+Business Media B.V., 2012. Vol. 842, 217-227 p.
Keyword [en]
In vivo, Microscopy, Micropipette, Rat, Mouse, Mucus, Stomach, Duodenum, Jejunum, Ileum, Colon
National Category
Physiology
Identifiers
URN: urn:nbn:se:uu:diva-170440DOI: 10.1007/978-1-61779-513-8_12OAI: oai:DiVA.org:uu-170440DiVA: diva2:509144
Available from: 2012-03-12 Created: 2012-03-12 Last updated: 2012-12-20Bibliographically approved

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Holm, LenaPhillipson, Mia

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