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Evaluation of the potential genotoxicity of chromium picolinate in mammalian cells in vivo and in vitro
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences, Toxicology. (Genetisk toxikologi)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences, Toxicology.
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2007 (English)In: Food and Chemical Toxicology, ISSN 0278-6915, E-ISSN 1873-6351, Vol. 45, no 7, 1097-1106 p.Article in journal (Refereed) Published
Abstract [en]

Chromium picolinate (CrPic) is a synthetic nutritional supplement primarily used for weight loss and muscle building. Recent studies have indicated that CrPic might be genotoxic and these findings together with the wide-spread consumer use, have increased the concern about its safety. In the present study we investigated the potential genotoxicity of CrPic in mice given a single intraperitoneal injection (up to 3 mg/kg b.wt.) by evaluating the frequency of micronucleated polychromatic erythrocytes (fMNPCE) in peripheral blood, and DNA damage in lymphocytes and hepatocytes. The fMNPCE was evaluated after 42 h and DNA damage after 16 h. Using the Comet assay DNA damage was also monitored in extended-term cultures of human lymphocytes and in L5178Y mouse lymphoma cells that had been exposed for 3 h to 500 μM CrPic under different exposure conditions.

A slight, but significant CrPic-induced increase in DNA damage (P < 0.001) was observed in the human lymphocytes, but only when these cells were exposed in the absence of serum. In all other experiments CrPic was found to be without genotoxic effects, both in vivo and in vitro. Taken together, our results suggest that a high concentration of CrPic might be DNA damaging, but only under non-physiological conditions.

Place, publisher, year, edition, pages
2007. Vol. 45, no 7, 1097-1106 p.
Keyword [en]
Chromium picolinate, Comet assay, Flow-cytometric micronucleus test, Mouse hepatocytes, Mouse bone marrow cells, Mouse and human lymphocytes, Mouse lymphoma cells
National Category
Pharmaceutical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-23155DOI: 10.1016/j.fct.2006.11.008ISI: 000247537800001OAI: oai:DiVA.org:uu-23155DiVA: diva2:50928
Available from: 2007-04-19 Created: 2007-04-19 Last updated: 2017-12-07Bibliographically approved

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Andersson, Maria A.Karlsson, Oskar MHellman, Björn E.

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