Entourage: the immune microenvironment following follicular lymphoma
2012 (English)In: Blood Cancer Journal, ISSN 2044-5385, Vol. 2, e52- p.Article in journal (Refereed) Published
In follicular lymphoma, nonmalignant immune cells are important. Follicular lymphoma depends on CD4+ cells, but CD8+ cells counteract it. We hypothesized that the presence of follicular lymphoma is associated with higher CD4+ than CD8+ cell numbers in the tumor microenvironment but not in the immune system. Using flow cytometry, pre-treatment and follow-up CD4/CD8 ratios were estimated in the bone marrow, blood and lymph nodes of untreated follicular lymphoma patients in two independent data sets (N-1 = 121; N-2 = 166). The ratios were analyzed for their relation with bone marrow lymphoma involvement. Bone marrows were also investigated with immunohistochemistry. In either data set, the bone marrow CD4/CD8 ratios were higher in bone marrows involved with lymphoma (P = 0.043 and 0.0002, respectively). The mean CD4/CD8 ratio was 1.0 in uninvolved and 1.4 in involved bone marrows. Also higher in involved bone marrows were CD4/CD56 and CD3CD25/CD3 ratios. No blood or lymph node ratios differed between bone marrow-negative and -positive patients. Sequential samples showed increased bone marrow CD4/CD8 ratios in all cases of progression to bone marrow involvement. Immunohistochemistry showed CD4+, CD57+, programmed death-1+, forkhead box protein 3+ and CD21+ cells accumulated inside the lymphoma infiltrates, whereas CD8+, CD56+ and CD68+ cells were outside the infiltrates. This study provides evidence in vivo that the microenvironment changes upon follicular lymphoma involvement.
Place, publisher, year, edition, pages
2012. Vol. 2, e52- p.
follicular lymphoma, microenvironment, bone marrow, CD4/CD8 ratio
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-170810DOI: 10.1038/bcj.2011.53ISI: 000300405300004OAI: oai:DiVA.org:uu-170810DiVA: diva2:509556