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Use of light and electron microscopy to study neuropharmacology ofneuroprotection
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Surgical Sciences, Anaesthesiology and Intensive Care.
2010 (English)In: Microscopy: Science, Technology, Applications and Education / [ed] Méndez-Vilas, A. & Díaz, J., Formatex , 2010, 40-51 p.Chapter in book (Refereed)
Abstract [en]

Use of light and electron microscopy in detecting neuroprotection induced by various drug treatments followingdifferentnoxious insults to the central nervous system (CNS) is still a new area of investigation. Research carried out in ourlaboratory since last 20 years show that microscopy is a good tool to study neuroprotection in the CNS caused by variouspharmacotherapeutic agents. Using light and transmission electron microscopy (TEM), it is possible to explore specificareas of brain exhibiting restoration or repair of neuronal, glial and endothelia cells after injury, a feature that is notfeasible to identify with any other biochemical, immunological or electrophysiological methods accurately. Thus,morphological changes in the CNS and their restoration by neuroprotective agents using microscopy are needed to supporttheir therapeutic efficacy in clinical situations to improve healthcare. Using immunohistochemistry, histology and TEM,our laboratory is engaged in finding new class of neuroprotective agents to reduce CNS pathology under wide variety ofneurological disorders. In this review, using morphological approaches, the therapeutic efficiency of some newneuroprotective agents in different CNS injury models are presented in support of our hypothesis. Our results show thatbrain or spinal cord trauma, psychostimulants abuse, exposure to heat stress and/or nanoparticles intoxication causesbreakdown of the blood-CNS barrier (BCNSB) in specific areas associated with brain pathology. This breakdown of theBCSNB and brain pathology could be significantly reduced by antioxidants, neurotrophic factors and antibodies toneurodestructive elements, e.g., serotonin (5-HT), nitric oxide synthase (NOS) or tumor necrosis factor alpha (TNF-α).Thus, restoration of BCNSB and reduction in the neuronal, glial or axonal damages are seen by these neuroprotectiveagents using light microscopy, immunohistochemistry and TEM. Taken together, our observations show that the use ofmicroscopy as a tool in exploring the neuroprotective efficacy of drugs or compounds in neuropharmacology ofneuroprotection is highly needed to improve quality of our healthcare system in neurological diseases.

Place, publisher, year, edition, pages
Formatex , 2010. 40-51 p.
, Microscopy Book Series, 4
Keyword [en]
immunohistochemistry, histology, light microscopy, transmission electronmicroscopy, blood-brain barrier, neuroprotection, nanoparticles, methamphetamine, morphine, neurotoxicity
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-171273OAI: oai:DiVA.org:uu-171273DiVA: diva2:510378
Available from: 2012-03-16 Created: 2012-03-16Bibliographically approved

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Sharma, Hari Shanker
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