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The Neurotransmitter ATP Triggers Ca2+ Responses Promoting Coordination of Pancreatic Islet Oscillations
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
2012 (English)In: Pancreas, ISSN 0885-3177, E-ISSN 1536-4828, Vol. 41, no 2, 258-263 p.Article in journal (Refereed) Published
Abstract [en]

Objectives: Pulsatile insulin release into the portal vein is critically dependent on entrainment of the islets in the pancreas into a common oscillatory phase. Because the pulses reflect periodic variations of the cytoplasmic Ca2+ concentration ([Ca2+](i)), we studied whether the neurotransmitters adenosine triphosphate (ATP) and acetylcholine promote synchronization of [Ca2+](i) oscillations between islets lacking contact.

Methods: Medium-sized and small mouse islets and cell aggregates were used for measuring [Ca2+](i) with the indicator fura-2.

Results: Exposure to acetylcholine resulted in an initial [Ca2+](i) peak followed by disappearance of the [Ca2+](i) oscillations induced by 11-mmol/L glucose. The effect of ATP was often restricted to an elusive [Ca2+](i) peak. The incidence of distinct [Ca2+](i) responses to ATP increased under conditions (accelerated superfusion, small islets, or cell aggregates) intended to counteract purinoceptor desensitization owing to intercellular accumulation of ATP. Attempts to imitate neural activity by brief (15 seconds) exposure to ATP or acetylcholine resulted in temporary synchronization of the glucose-induced [Ca2+](i) oscillations between islets lacking contact.

Conclusions: The data support the idea that purinergic signaling has a key role for coordinating the oscillatory activity of the islets in the pancreas, reinforcing previous arguments for the involvement of nonadrenergic, noncholinergic neurons.

Place, publisher, year, edition, pages
2012. Vol. 41, no 2, 258-263 p.
Keyword [en]
acetylcholine, ATP, cytoplasmic calcium, islets of langerhans, purinoceptor desensitization, synchronization
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-171411DOI: 10.1097/MPA.0b013e3182240586ISI: 000300641500014OAI: oai:DiVA.org:uu-171411DiVA: diva2:511115
Available from: 2012-03-20 Created: 2012-03-19 Last updated: 2017-12-07Bibliographically approved

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Gylfe, ErikGrapengiesser, EvaDansk, HeléneHellman, Bo

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