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Cross-species amplification and development of microsatellites for six species of European Coenagrionid damselflies
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Population and Conservation Biology.
2012 (English)In: Conservation Genetics Resources, ISSN 1877-7252, Vol. 4, no 1, 191-196 p.Article in journal (Refereed) Published
Abstract [en]

We describe the cross-amplification and development of new loci for six species of closely related European damselflies. First, twenty-nine published microsatellites for the damselflies Coenagrion puella and C. mercuriale were multiplexed using M13-tagged primers, tested on 23 individuals, and then cross-species amplified on 21-26 individuals of C. armatum, C. johanssoni, C. pulchellum and C. scitulum. Second, sixteen new primers were developed for use in C. armatum, C. johanssoni and C. scitulum, and screened on 21 individuals. Values for observed heterozygosities and number of alleles ranged between 0.00-0.87 and 2-19 respectively (over all loci and species). For all species the tested loci provide a minimum of 1-8 usable markers for population genetic studies.

Place, publisher, year, edition, pages
2012. Vol. 4, no 1, 191-196 p.
Keyword [en]
Coenagrion, Damselfly, Microsatellite, Cross-species amplification
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-171659DOI: 10.1007/s12686-011-9506-4ISI: 000300772500046OAI: oai:DiVA.org:uu-171659DiVA: diva2:512290
Available from: 2012-03-27 Created: 2012-03-25 Last updated: 2012-03-27Bibliographically approved

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