uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Automated sample preparation method for mass spectrometry analysis on recombinant proteins
KTH, Proteomik.
KTH, Proteomik.
KTH, Proteomik.
KTH, Proteomik.
Show others and affiliations
2009 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1216, no 20, 4457-4464 p.Article in journal (Refereed) Published
Abstract [en]

A completely automated procedure for the purification and desalting of proteins with a polyhistidine purification tag prior to mass spectrometry analysis is presented. The system is ideal for rapid quality control and optimization studies and it provides researchers with a straightforward, reliable tool for studies of recombinant proteins. Forty-eight samples can be prepared within 4.5 h and only small cultivation and buffer volumes are needed. In this proof of concept, 19,000-35,000 Da recombinant proteins from both crude and clarified cell lysates were successfully prepared for subsequent analysis by electrospray ionization anti matrix-assisted laser desorption/ionization mass spectrometry as well as by gel electrophoresis.

Place, publisher, year, edition, pages
2009. Vol. 1216, no 20, 4457-4464 p.
Keyword [en]
Mass spectrometry, High-throughput, Automated sample preparation, Recombinant proteins, IMAC, RPLC, affinity-chromatography, purification
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-172141DOI: 10.1016/j.chroma.2009.03.041ISI: 000265956100021OAI: oai:DiVA.org:uu-172141DiVA: diva2:513427
Note
QC 20100525Available from: 2010-08-05 Created: 2012-04-02 Last updated: 2017-12-07

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Steen, JohannaRamström, MargaretaUhlén, MathiasHober, SophiaOttosson, Jenny

Search in DiVA

By author/editor
Steen, JohannaRamström, MargaretaUhlén, MathiasHober, SophiaOttosson, Jenny
In the same journal
Journal of Chromatography A
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 656 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf