Construction and deep sequencing of cDNA libraries representing medium-sized RNA
(English)Manuscript (preprint) (Other academic)
Transcriptome analysis has recently become feasible with the application of RNA-seq, the high-throughput sequencing analysis of RNAs through cDNA synthesis. Current transcriptome analysis approaches are limited to analysis of long (>200 nts) or short (~20 nts) RNAs. Here we describe an approach that can be used for the analysis of the medium-sized (~200 nts) RNA transcriptome, which reveals the full-length sequence of RNAs at the single molecule level. Our approach can easily be adapted to generate libraries that are suitable for sequencing by several of the currently used next-generation platforms.
high-throughput sequencing, 454, snRNA, cDNA library
Natural Sciences Biochemistry and Molecular Biology
Research subject Molecular Cellbiology
IdentifiersURN: urn:nbn:se:uu:diva-172422OAI: oai:DiVA.org:uu-172422DiVA: diva2:514629