The demonstration in rat liver cell sap of protein kinase and phosphoprotein phosphatase active on fructose-bisphosphatase
1982 (English)In: Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology, ISSN 0167-4838, Vol. 706, no 2, 239-244 p.Article in journal (Refereed) Published
A protein kinase active on fructose-bisphosphatase (D-fructose-1,6-bisphosphate 1-phosphohydrolase, EC 184.108.40.206) was demonstrated in rat liver cell sap. The protein kinase activity was stimulated by cyclic AMP and coincided with the activity of cyclic AMP-dependent protein kinase type I. In addition, three different peaks of phosphoprotein phosphatase active on [32P] phosphofructose-bisphosphatase were found on chromatography of rat liver cell sap on a DEAE-cellulose column. These phosphatases needed divalent cations for full activity. 5'-AMP, a negative modulator of fructose-bisphosphatase, had no effect on the phosphorylation-de-phosphorylation reactions of the enzyme. ATP and Ca2+ did not influence the dephosphorylation reaction of fructose-bisphosphatase.
Place, publisher, year, edition, pages
1982. Vol. 706, no 2, 239-244 p.
fructose-bisphosphatase, protein kinase, phosphoprotein phosphatase
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-172449DOI: 10.1016/0167-4838(82)90492-7OAI: oai:DiVA.org:uu-172449DiVA: diva2:514697