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In-vivo extravasation induces the expression of interleukin 1 receptor type 1 in human neutrophils
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , UCR-Uppsala Clinical Research Center.
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2012 (English)In: Clinical and Experimental Immunology, ISSN 0009-9104, E-ISSN 1365-2249, Vol. 168, no 1, 105-112 p.Article in journal (Refereed) Published
Abstract [en]

In order to address neutrophil activation during inflammation we assessed the expression of interleukin 1 receptor type 1 (IL-1R1) following in-vivo extravasation. Extravasated neutrophils were collected from 11 healthy study subjects by a skin chamber technique and compared to neutrophils in peripheral blood. Expression of IL-1R1 was assessed by microarray, quantitative polymerase chain reaction (qPCR), Western blot, flow cytometry, enzyme linked immunosorbent assay (ELISA) and immunoelectron microscopy (iEM). IL-1R1 was induced following extravasation, demonstrated by both gene array and qPCR. Western blot demonstrated an increased expression of IL-1R1 in extravasated leucocytes. This was confirmed further in neutrophils by flow cytometry and iEM that also demonstrated an increased intracellular pool of IL-1R1 that could be mobilized by N-formyl-methionine-leucine-phenylalanine (fMLP). Stimulation of peripheral neutrophils with IL-1 resulted in transcription of NF?B and a number of downstream chemokines and the corresponding chemokines were also induced following in-vivo extravasation. The present results demonstrate that IL-1R1 is induced following extravasation and exists on the neutrophil surface, as well as in a mobile intracellular pool. Furthermore, neutrophils express functional IL-1R1 as demonstrated by the induction of chemokines following IL-1 stimulation. The results indicate a potential role for IL-1 in the activation of neutrophils at inflammatory sites.

Place, publisher, year, edition, pages
2012. Vol. 168, no 1, 105-112 p.
Keyword [en]
extravasation, IL-1R1, neutrophils
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-172813DOI: 10.1111/j.1365-2249.2011.04548.xISI: 000300974800016OAI: oai:DiVA.org:uu-172813DiVA: diva2:516347
Available from: 2012-04-18 Created: 2012-04-16 Last updated: 2012-04-18Bibliographically approved

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Held, Claes
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