Comparison of real-time PCR and pyrosequencing for typing Bordetella pertussis toxin subunit 1 variants
2006 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 65, no 1, 153-158 p.Article in journal (Refereed) Published
We describe two newly developed methods for rapid typing of the pertussis toxin subunit 1 gene (ptxS1). A real-time PCR assay based on hybridization probes and a Pyrosequencing assay were developed and the specificity, sensitivity, cost, hands-on time and post-assay data processing were compared to Sanger sequencing. Both methods enabled discrimination of all four allelic variants, correctly identified all ptxS1 alleles of 143 strains tested and proved suitable for large-scale screening of B. pertussis strains.
Place, publisher, year, edition, pages
2006. Vol. 65, no 1, 153-158 p.
Alleles, Bordetella pertussis/classification/*genetics, DNA; Bacterial/chemistry/genetics, Humans, Pertussis Toxin/chemistry/*genetics, Pertussis Vaccine/genetics, Polymerase Chain Reaction, Polymorphism; Single Nucleotide, Sequence Analysis; DNA, Whooping Cough/microbiology
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-23881DOI: 10.1016/j.mimet.2005.07.002PubMedID: 16095736OAI: oai:DiVA.org:uu-23881DiVA: diva2:51655