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Comparison of real-time PCR and pyrosequencing for typing Bordetella pertussis toxin subunit 1 variants
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences.
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2006 (English)In: Journal of Microbiological Methods, ISSN 0167-7012, E-ISSN 1872-8359, Vol. 65, no 1, 153-158 p.Article in journal (Refereed) Published
Abstract [en]

We describe two newly developed methods for rapid typing of the pertussis toxin subunit 1 gene (ptxS1). A real-time PCR assay based on hybridization probes and a Pyrosequencing assay were developed and the specificity, sensitivity, cost, hands-on time and post-assay data processing were compared to Sanger sequencing. Both methods enabled discrimination of all four allelic variants, correctly identified all ptxS1 alleles of 143 strains tested and proved suitable for large-scale screening of B. pertussis strains.

Place, publisher, year, edition, pages
2006. Vol. 65, no 1, 153-158 p.
Keyword [en]
Alleles, Bordetella pertussis/classification/*genetics, DNA; Bacterial/chemistry/genetics, Humans, Pertussis Toxin/chemistry/*genetics, Pertussis Vaccine/genetics, Polymerase Chain Reaction, Polymorphism; Single Nucleotide, Sequence Analysis; DNA, Whooping Cough/microbiology
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-23881DOI: 10.1016/j.mimet.2005.07.002PubMedID: 16095736OAI: oai:DiVA.org:uu-23881DiVA: diva2:51655
Available from: 2007-03-01 Created: 2007-03-01 Last updated: 2010-05-26Bibliographically approved

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Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=16095736&dopt=Citation

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Storm, MartinBondeson, Kåre
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