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Phospholipid- dependent and EGTA-inhibited protein kinase from maize seedlings
Polish Academy of Science, Warsawa. (Grazyna Muszynska)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical and Physiological Chemistry. (ek pia)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical and Physiological Chemistry. (engström lorentz)
1993 (English)In: Biochemistry and Molecular Biology International, ISSN 1039-9712, Vol. 30, no 5, 849-860 p.Article in journal (Refereed) Published
Abstract [en]

Chromatography of a maize seedling extract on DEAE-cellulose, followed by Octyl-Sepharose yielded a fraction with protein kinase activity which was stimulated by phosphatidylserine plus diolein. The activity was not enhanced by calcium ions but was inhibited by chelating agents and could then be restored by the addition of calcium ions. All these facts indicated that the maize protein kinase was similar to mammalian protein kinase C. The maize enzyme phosphorylated myelin basic protein (MBP) and histone H1, but the MBP-peptide4-14 and protamine were poor substrates for the enzyme. Further purification of the enzyme fraction followed by phosphorylation and SDS-polyacrylamide gel electrophoresis, revealed two labeled bands of Mw 59 and 83 kDa the former of which probably being the protein kinase C.

Place, publisher, year, edition, pages
1993. Vol. 30, no 5, 849-860 p.
Keyword [en]
myelin basic protein, phorbol 12-myristate 13-acetate
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-173133PubMedID: 7693122OAI: oai:DiVA.org:uu-173133DiVA: diva2:516708
Available from: 2012-04-19 Created: 2012-04-19 Last updated: 2017-12-07Bibliographically approved

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