D-amino acid residues as substrate specificity determinants for casein kinase II
1994 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 200, no 3, 1564-1569 p.Article in journal (Refereed) Published
A set of diastereomeric peptides RRRDDDSDDD each with a corresponding D-amino acid residue successively in every position (except the arginines) was tested as substrates for casein kinase II. It was found that the D-serine containing peptide was not detectably phosphorylated. The replacements at the positions +1, -1 and +2 decreased the kII values more than 60, 30 and 20 times, respectively. The effect of the L/D replacements decreased with increased distance from the serine. The D-amino acid scan used herein seems to be a helpful complementary tool for studies of substrate specificity determinants for different protein kinases.
Place, publisher, year, edition, pages
1994. Vol. 200, no 3, 1564-1569 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-173141DOI: 10.1006/bbrc.1994.1629OAI: oai:DiVA.org:uu-173141DiVA: diva2:516718