Quantification of subnanomolar amounts of phosphate bound to seryl and threonyl residues in phosphoproteins using alkaline hydrolysis and malachite green
1993 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 214, no 1, 138-141 p.Article in journal (Refereed) Published
An assay for protein-bound phosphate with a capacity to determine 100 pmol of phosphate is described. It is based on the combination of two well known methods: the alkaline hydrolysis of phosphate from seryl and threonyl residues in phosphoproteins and the quantification of the released phosphate by the use of malachite green and phosphomolybdate.
Place, publisher, year, edition, pages
1993. Vol. 214, no 1, 138-141 p.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-173189DOI: 10.1006/abio.1993.1468OAI: oai:DiVA.org:uu-173189DiVA: diva2:516838