Conditional DC depletion does not affect priming of encephalitogenic Th cells in EAE
2012 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 42, no 10, 2555-2563 p.Article in journal (Refereed) Published
EAE, an animal model for multiple sclerosis, is a Th17- and Th1-cell-mediated auto-immune disease, but the mechanisms leading to priming of encephalitogenicTcells in autoimmune neuroinflammation are poorly understood. To investigate the role of dendritic cells (DCs) in the initiation of autoimmuneTh17- andTh1-cell responses andEAE, we used mice transgenic for a simian diphtheria toxin receptor (DTR) expressed under the control of the murineCD11c promoter (CD11c-DTRmice onC57BL/6 background).EAEwas induced by immunization with myelin oligodendrocyte glycoprotein (MOG) protein in CFA. DCs were depleted on the day before and 8 days afterMOG immunization. The mean clinicalEAEscore was only mildly reduced inDC-depleted mice when DCs were ablated beforeEAEinduction. The frequency of activatedTh cells was not altered, andMOG-inducedTh17 orTh1-cell responses were not altered, in the spleens ofDC-depleted mice. Similar results were obtained ifDCswere ablated the first 10 days afterMOGimmunization with repeatedDCdepletions. Unexpectedly, transient depletion of DCs did not affect priming or differentiation of MOG-inducedTh17 andTh1-cell responses or the incidence ofEAE. Thus, the mechansim of priming ofTh cells inEAEremains to be elucidated.
Place, publisher, year, edition, pages
2012. Vol. 42, no 10, 2555-2563 p.
Immunology in the medical area
IdentifiersURN: urn:nbn:se:uu:diva-173266DOI: 10.1002/eji.201142239ISI: 000309610200004PubMedID: 22806332OAI: oai:DiVA.org:uu-173266DiVA: diva2:517516
De två första författarna delar förstaförfattarskapet.2012-04-232012-04-212012-11-26Bibliographically approved