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Detection of recombinant human EPO administered to horses using MAIIA lateral flow isoform test
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
L.C.H., Laboratoire des Courses Hippiques, France.
L.C.H., Laboratoire des Courses Hippiques, France.
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2012 (English)In: Analytical and Bioanalytical Chemistry, ISSN 1618-2642, E-ISSN 1618-2650, Vol. 403, no 6, 1619-1628 p.Article in journal (Refereed) Published
Abstract [en]

Doping of horses with recombinant human erythropoietin (rHuEPO) to illegally enhance their endurance capacity in horseracing has been reported during the last years. This leads to increased blood viscosity which can result in sudden death and is of concern for the horse welfare. Additionally, the horse can start production of rHuEPO antibodies, which cross-reacts with endogenous equine EPO and can lead to severe anaemia and even death. In this study, a novel micro-chromatographic method, EPO WGA MAIIA, has been tested for the capability in plasma and urine samples to detect administration of erythropoiesis-stimulating agents, like the rHuEPO glycoprotein varieties Eprex and Aranesp, to horses. After administration of 40 IU Eprex kg −1 day −1 to seven horses during 6 days, the presence of Eprex in horse plasma was detected up to 2–5 days after last injection. In urine samples collected from two horses, Eprex was detected up to 3 days. A single injection of Aranesp (0.39 μg/kg) was detected up to 9 days in plasma and up to 8 days, the last day of testing, in the urine sample. The LC-FAIMS-MS/MS system, with 1 day reporting time, confirmed the presence of Eprex up to 1 day after last injection for six out of seven horses and the presence of Aranesp up to 5 days after last injection in plasma samples. The MAIIA system showed to be a promising tool with high sensitivity and extremely short reporting time (1 h).

Place, publisher, year, edition, pages
2012. Vol. 403, no 6, 1619-1628 p.
Keyword [en]
Aranesp, EPO doping control, Eprex, Equine EPO, Micro-chromatography, WGA
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-173870DOI: 10.1007/s00216-012-5972-0ISI: 000304166500014OAI: oai:DiVA.org:uu-173870DiVA: diva2:525437
Available from: 2012-05-08 Created: 2012-05-08 Last updated: 2012-06-11Bibliographically approved

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Lönnberg, MariaBondesson, Ulf
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