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Surface morphology and adsorbed proteins affect phagocyte responses to nano-porous alumina
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Surface Biotechnology. Department of Physical and Analytical Chemistry, Surface Biotechnology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Physical and Analytical Chemistry.
2006 (Swedish)In: J Mater Sci: Mater Med, Vol. 17, 1101–1111- p.Article in journal (Refereed) Published
Abstract [en]

This study evaluates human neutrophil responses

to aluminum oxide membranes with different pore sizes

(20 nm and 200 nm in diameter) uncoated and pre-coated

with serum, collagen I, or fibrinogen. The effect of released

neutrophil granule components on the survival of osteoblastic

cells (MG63) bound to the alumina membranes has also

been evaluated.Without protein coatings the 20 nm pore-size

membranes prompt higher reactive oxygen species (ROS)

production as assessed by luminol-amplified chemiluminescence

than the 200 nm pore-size membranes. Such pore-size

depending responses were also found on membranes precoated

with fibrinogen, but not with collagen or serum were

in fact a much lower ROS production was observed. In addition,

uncoated and fibrinogen-coated membranes prompt

stronger release of the granule enzymes, myeloperoxidase

and elastase, than collagen or serum-coated alumina. Equally

important, we found that surface-mediated phagocyte activation

and the subsequent release of granule components had a

significant affect on the adhesion, viability and proliferation

of osteoblasts. This stresses the importance of studying not

only cell/surface interactions but also cell/cell interactions in

wound healing and tissue regeneration processes.

Place, publisher, year, edition, pages
2006. Vol. 17, 1101–1111- p.
Identifiers
URN: urn:nbn:se:uu:diva-24813OAI: oai:DiVA.org:uu-24813DiVA: diva2:52587
Available from: 2007-02-12 Created: 2007-02-12 Last updated: 2011-01-11

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