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Identification of a novel protein kinase Cδ-Smac complex that dissociates during paclitaxel-induced cell death
Lund University, Center for Molecular Pathology, Skåne University Hospital, Malmö, Sweden.
Lund University, Center for Molecular Pathology, Skåne University Hospital, Malmö, Sweden.
Lund University, Center for Molecular Pathology, Skåne University Hospital, Malmö, Sweden.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Medicinska och farmaceutiska vetenskapsområdet, centrumbildningar mm , Ludwig Institute for Cancer Research.
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2012 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 586, no 8, 1166-1172 p.Article in journal (Refereed) Published
Abstract [en]

Protein kinase C (PKC) δ is a regulator of apoptosis with both pro- and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKCδ. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKCδ rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKCδ when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKCδ by associating with Smac may prevent its apoptotic effects.

STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: PKC deltaphysically interacts with SMAC by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3, 4) XIAPphysically interacts with SMAC by anti tag coimmunoprecipitation (View interaction).

Place, publisher, year, edition, pages
2012. Vol. 586, no 8, 1166-1172 p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-174128DOI: 10.1016/j.febslet.2012.03.033ISI: 000303106100013PubMedID: 22465666OAI: oai:DiVA.org:uu-174128DiVA: diva2:526533
Available from: 2012-05-14 Created: 2012-05-14 Last updated: 2017-12-07Bibliographically approved

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