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Lipid-Based Passivation in Nanofluidics
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Computational and Systems Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.
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2012 (English)In: Nano letters (Print), ISSN 1530-6984, E-ISSN 1530-6992, Vol. 12, no 5, 2260-2265 p.Article in journal (Refereed) Published
Abstract [en]

Stretching DNA in nanochannels is a useful tool for direct, visual studies of genomic DNA at the single molecule level. To facilitate the study of the interaction of linear DNA with proteins in nanochannels, we have implemented a highly effective passivation scheme based on lipid bilayers. We demonstrate virtually complete long-term passivation of nanochannel surfaces to a range of relevant reagents, including streptavidin-coated quantum dots, RecA proteins, and RecA-DNA complexes. We show that the performance of the lipid bilayer is significantly better than that of standard bovine serum albumin-based passivation. Finally, we show how the passivated devices allow us to monitor single DNA cleavage events during enzymatic degradation by DNase I. We expect that our approach will open up for detailed, systematic studies of a wide range of protein-DNA interactions with high spatial and temporal resolution.

Place, publisher, year, edition, pages
2012. Vol. 12, no 5, 2260-2265 p.
Keyword [en]
Nanofluidics, passivation, antifouling, lipid bilayer, protein-DNA interactions, single molecules
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-175207DOI: 10.1021/nl204535hISI: 000303696400015OAI: oai:DiVA.org:uu-175207DiVA: diva2:530957
Available from: 2012-06-05 Created: 2012-06-04 Last updated: 2012-06-05Bibliographically approved

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Persson, Fredrik
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