uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Influences on gene expression in vivo by a Shine-Dalgarno sequence.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, The Linnaeus Centre for Bioinformatics. (Ardell)
Show others and affiliations
2006 (English)In: Mol Microbiol, ISSN 0950-382X, Vol. 60, no 2, 480-92 p.Article in journal (Other scientific) Published
Abstract [en]

The Shine-Dalgarno (SD+: 5'-AAGGAGG-3') sequence anchors the mRNA by base

pairing to the 16S rRNA in the small ribosomal subunit during translation initiation. We have here compared

how an SD+ sequence influences gene expression, if located upstream or downstream of an initiation codon.

The positive effect of an upstream SD+ is confirmed. A downstream SD+ gives decreased gene expression.

This effect is also valid for appropriately modified natural Escherichia coli genes. If an SD+ is placed

between two potential initiation codons, initiation takes place predominantly at the second start site.

The first start site is activated if the distance between this site and the downstream SD+ is enlarged

and/or if the second start site is weakened. Upstream initiation is eliminated if a stable stem-loop

structure is placed between this SD+ and the upstream start site. The results suggest that the two start

sites compete for ribosomes that bind to an SD+ located between them. A minor positive contribution to

upstream initiation resulting from 3' to 5' ribosomal diffusion along the mRNA is suggested. Analysis

of the E. coli K12 genome suggests that the SD+ or SD-like sequences are systematically avoided in the

early coding region suggesting an evolutionary significance.

Place, publisher, year, edition, pages
2006. Vol. 60, no 2, 480-92 p.
Keyword [en]
Base Sequence, Binding Sites, Codon; Initiator/genetics/metabolism, Escherichia coli/*genetics/metabolism, Gene Expression Regulation; Bacterial, Genes; Bacterial/genetics, Genes; Reporter, Molecular Sequence Data, Nucleic Acid Conformation, Protein Biosynthesis/*genetics, RNA; Bacterial/metabolism, RNA; Messenger/metabolism, RNA; Ribosomal; 16S/metabolism, RNA; Transfer; Amino Acyl/chemistry/metabolism, Ribosomes/*metabolism
URN: urn:nbn:se:uu:diva-25430PubMedID: 16573696OAI: oai:DiVA.org:uu-25430DiVA: diva2:53204
Available from: 2007-02-12 Created: 2007-02-12 Last updated: 2011-01-11

Open Access in DiVA

No full text

Other links

By organisation
The Linnaeus Centre for Bioinformatics

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 177 hits
ReferencesLink to record
Permanent link

Direct link