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In situ proximity ligation assay for microscopy and flow cytometry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools.
2011 (English)In: Current Protocols in Cytometry, ISSN 1934-9297, Vol. Suppl 56, Unit 9.36- p.Article in journal (Refereed) Published
Abstract [en]

The ability to study proteins and protein interactions inside cells and tissues is important for elucidating how cells function in health and disease. The in situ proximity ligation assay (in situ PLA) presented here can be used to visualize proteins, protein-protein interactions, and post-translational modifications in cells and tissues. The method is based upon the use of antibodies that target the proteins involved in an interaction; hence, the method has the advantage that it can be used in clinical specimens, providing localized, quantifiable single molecule detection in single cells. This unit describes how in situ PLA can be used with fluorescence microscopy and flow cytometry to study proteins (obtaining high sensitivity and specificity of detection) and protein interactions. It also includes information on expected results and information on how to troubleshoot the assay.

Place, publisher, year, edition, pages
2011. Vol. Suppl 56, Unit 9.36- p.
National Category
Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-175742DOI: 10.1002/0471142956.cy0936s56OAI: oai:DiVA.org:uu-175742DiVA: diva2:532805
Available from: 2012-06-12 Created: 2012-06-12 Last updated: 2013-03-13Bibliographically approved

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Söderberg, Ola

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