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Controlled-pH Tissue Cleanup Protocol for Signal Enhancement of Small Molecule Drugs Analyzed by MALDI-MS Imaging
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. (Medicinsk masspektrometri)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. (Medicinsk masspektrometri)
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2012 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 84, no 10, 4603-4607 p.Article in journal (Refereed) Published
Abstract [en]

The limit of detection of low-molecular weight compounds in tissue sections, analyzed by matrix assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI), was significantly improved by employing sample washing using a pH-controlled buffer solution. The pH of the washing solutions were set at values whereby the target analytes would have low solubility. Washing the tissue sections in the buffered solution resulted in removal of endogenous soluble ionization-suppressing compounds and salts, while the target compound remained in situ with minor or no delocalization during the buffered washing procedure. Two pharmaceutical compounds (cimetidine and imipramine) and one new protease inhibitor compound were successfully used to evaluate the feasibility of the pH-controlled tissue washing protocol for MALDI-MSI. Enhancement in signal-to-noise ratio was achieved by a factor of up to 10.

Place, publisher, year, edition, pages
2012. Vol. 84, no 10, 4603-4607 p.
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Chemical Sciences
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URN: urn:nbn:se:uu:diva-175615DOI: 10.1021/ac203322qISI: 000303965500050OAI: oai:DiVA.org:uu-175615DiVA: diva2:532844
Available from: 2012-06-12 Created: 2012-06-11 Last updated: 2017-12-07Bibliographically approved

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Shariatgorji, MohammadrezaKällback, PatrikGoodwin, Richard J. A.Andrén, Per E.

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