uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Maturation of in vitro-generated human islets after transplantation in nude mice
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Oncology, Radiology and Clinical Immunology, Clinical Immunology.
Show others and affiliations
2007 (English)In: Molecular and Cellular Endocrinology, ISSN 0303-7207, E-ISSN 1872-8057, Vol. 264, no 1-2, 28-34 p.Article in journal (Refereed) Published
Abstract [en]

The long-term function of human pancreatic islet grafts may depend on the neogenesis of beta cells from epithelial precursors within the grafted tissue. We have developed an in vitro model for human islet neogenesis. In this study, we have investigated the morphological signs of maturation in cultivated human islet buds (CHIBs) before and after transplantation. Clusterin is a molecule associated with beta-cell differentiation in rodents. In adult human islets, clusterin expression was located only in alpha- and PP-cells, but in CHIBs and human fetal islets, it was distributed in all four types of endocrine cells. Some immature endocrine cells in the CHIBs co-expressed insulin and glucagon. After transplantation, CHIBs became mature with one type of hormone per endocrine cell, and clusterin expression became restricted in alpha-cells. Cells co-expressing endocrine markers and cytokeratin 19, as a sign of ductal to endocrine cell transition, were frequently detected in both fresh islets and CHIBs after transplantation. We conclude that clusterin may be involved in the development of islets, and the in vitro-derived islets become mature after transplantation into nude mice. Ductal cell differentiation into endocrine cells may be an important factor in sustaining the long-term function of islet transplants.

Place, publisher, year, edition, pages
2007. Vol. 264, no 1-2, 28-34 p.
Keyword [en]
Islet, Neogenesis, Maturation, Transplantation
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-25736DOI: 10.1016/j.mce.2006.10.007ISI: 000244157800004PubMedID: 17116362OAI: oai:DiVA.org:uu-25736DiVA: diva2:53510
Available from: 2007-02-13 Created: 2007-02-13 Last updated: 2011-02-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMedhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=17116362&dopt=Citation

Search in DiVA

By author/editor
Korsgren, Olle
By organisation
Clinical Immunology
In the same journal
Molecular and Cellular Endocrinology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 273 hits
ReferencesLink to record
Permanent link

Direct link