uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Residue-level elucidation of the ligand-induced protein binding on phenyl-argarose microspheres by NMR hydrogen/deuterium exchange technique
Show others and affiliations
2012 (English)In: Soft Matter, ISSN 1744-683X, Vol. 8, no 23, 6248-6255 p.Article in journal (Refereed) Published
Abstract [en]

Protein-ligand interactions on liquid-solid interfaces governed the design of functional biomaterials. However, accurate residue details of ligand induced protein binding and unfolding on an interface were still unknown by the current ensemble of protein structure characterizations. Here, a hydrogen/deuterium (H/D) approach coupled with analysis of NMR TOCSY spectra and the solvent accessible surface area (SASA) was designed to enable residue level understanding of lysozyme adsorbed at a phenyl-ligand modified surface. Results showed that the binding sites and unfolding of lysozyme molecules on phenyl-agarose microspheres demonstrated significant ligand-density dependence and protein-coverage dependence. Either increasing ligand density or decreasing adsorption coverage would lead to more binding sites and unfolding of the protein molecules. With the multipoint adsorption strengthening, the protein molecule changed from lying end-on to side-on. Finally, Molecular Dock simulation was utilized to evaluate the NMR determined binding sites based on energy ranking of the binding. It confirmed that this NMR approach represents a reliable route to in silico abundant residue-level structural information during protein interaction with biomaterials.

Place, publisher, year, edition, pages
2012. Vol. 8, no 23, 6248-6255 p.
National Category
Chemical Sciences
URN: urn:nbn:se:uu:diva-176451DOI: 10.1039/c2sm25117eISI: 000304309300009OAI: oai:DiVA.org:uu-176451DiVA: diva2:535644
Available from: 2012-06-20 Created: 2012-06-19 Last updated: 2012-06-20Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Janson, Jan-Christer
By organisation
Analytical Chemistry
In the same journal
Soft Matter
Chemical Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 394 hits
ReferencesLink to record
Permanent link

Direct link