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Differentiation of mast cell subpopulations from mouse embryonic stem cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools. Uppsala University, Science for Life Laboratory, SciLifeLab.
2012 (English)In: JIM - Journal of Immunological Methods, ISSN 0022-1759, E-ISSN 1872-7905, Vol. 382, no 1-2, 160-166 p.Article in journal (Refereed) Published
Abstract [en]

Mast cells can generally be divided into two major groups, connective tissue mast cells and mucosal mast cells. We and others have previously shown that these mast cell populations can be developed in vitro from mouse bone marrow stem cells using a combination of specific growth factors and cytokines. Mast cell differentiation from mouse embryonic stem (ES) cells is an important alternative method when developing mast cells from an embryonic lethal genetic deficiency or to reduce the use and handling of experimental animals. In this study, we have used protocols prior known to induce connective tissue like mast cells (CTLMC) (SCF and IL-4) and mucosal like mast cells (MLMC) (SCF, IL-3, IL-9 and TGF-β) from mouse bone marrow progenitor cells and employed these protocols to study if phenotype specific mast cells can be developed from ES cells. We here demonstrate that mast cells of the different phenotypes, CTLMC and MLMC, can be derived from mouse ES cells. The mast cell populations were characterized by chymase expression, receptor expression and their difference in activation pattern and in activation-induced survival.

Place, publisher, year, edition, pages
2012. Vol. 382, no 1-2, 160-166 p.
Keyword [en]
cytosolic diffusion, single particle tracking, photoactivated localization microscopy, stroboscopic illumination
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-176860DOI: 10.1016/j.jim.2012.05.020ISI: 000307088700016PubMedID: 22683543OAI: oai:DiVA.org:uu-176860DiVA: diva2:537254
Available from: 2012-06-26 Created: 2012-06-26 Last updated: 2012-10-01Bibliographically approved

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Ullerås, Erik
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Molecular toolsScience for Life Laboratory, SciLifeLab
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