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Synthetic biology challenges long-held hypotheses in translation, codon bias and transcription
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Structure and Molecular Biology.
2012 (English)In: Biotechnology Journal, ISSN 1860-6768, E-ISSN 1860-7314, Vol. 7, no 7, 835-845 p.Article, review/survey (Refereed) Published
Abstract [en]

Synthetic biology is a powerful experimental approach, not only for developing new biotechnology applications, but also for testing hypotheses in basic biological science. Here, examples from our research using the best model system, Escherichia coli, are reviewed. New evidence drawn from synthetic biology has overturned several long-standing hypotheses regarding the mechanisms of transcription and translation: (i) all native aminoacyl-tRNAs are not equally efficient in translation at equivalent concentrations; (ii) accommodation is not always rate limiting in translation, and may not be for any aminoacyl-tRNA; (iii) proline is the only N-alkyl-amino acid in the genetic code not because of special suitability for protein structure, but because of its comparatively high nucleophilicity; (iv) the usages of most sense codons in E. coli do not correlate with cognate tRNA abundances and (v) class II transcriptional pausing and termination by T7 RNA polymerase cannot be assumed to occur in vivo based on in vitro data. Implications of these conclusions for the biotechnology field are discussed.

Place, publisher, year, edition, pages
2012. Vol. 7, no 7, 835-845 p.
Keyword [en]
Aminoacyl-tRNA, Codon bias, Ribosome, Transcription termination, Translation
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:uu:diva-178186DOI: 10.1002/biot.201200002ISI: 000305888000009OAI: oai:DiVA.org:uu-178186DiVA: diva2:542281
Available from: 2012-07-31 Created: 2012-07-30 Last updated: 2017-12-07Bibliographically approved

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Forster, Anthony C.

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