Structural and biochemical characterization of compounds inhibiting Mycobacterium tuberculosis Pantothenate Kinase
2013 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 288, no 25, 18260-18270 p.Article in journal (Refereed) Published
Mycobacterium tuberculosis, the bacterial causative agent oftuberculosis, currently affects millions of people. The emergence of drug-resistant strains makes development of new antibiotics targeting the bacterium a global health priority. Pantothenate kinase, a key enzyme in the universal biosynthesis of the essential cofactor CoA, was targeted in this study to find new tuberculosis drugs. The biochemicalcharacterizations of two new classes of compounds that inhibitpantothenate kinase from M. tuberculosis are described, along with crystal structures of their enzyme-inhibitor complexes. These represent the first crystal structures of this enzyme with engineered inhibitors. Both classes of compounds bind in the active site of the enzyme, overlapping with the binding sites of the natural substrate and product, pantothenateand phosphopantothenate, respectively. One class of compounds also interferes with binding of the cofactor ATP. The complexes were crystallized in two crystal forms, one of which is in a new space group for this enzyme and diffracts to the highest resolution reported for anypantothenate kinase structure. These two crystal forms allowed, for the first time, modeling of the cofactor-binding loop in both open and closed conformations. The structures also show a binding mode of ATP different from that previously reported for the M. tuberculosis enzyme but similar to that in the pantothenate kinases of other organisms.
Place, publisher, year, edition, pages
2013. Vol. 288, no 25, 18260-18270 p.
Tuberculosis, Mycobacterium tuberculosis, drug development, pantothenate kinase, PanK
Structural Biology Biochemistry and Molecular Biology
Research subject Molecular Biology; Biochemistry
IdentifiersURN: urn:nbn:se:uu:diva-179056DOI: 10.1074/jbc.M113.476473ISI: 000320721900030OAI: oai:DiVA.org:uu-179056DiVA: diva2:543169
FunderSwedish Research Council