Development of a suit of microsatellite markers for the southern anteater-chat Myrmecocichla formicivora.
(English)Manuscript (preprint) (Other academic)
We tested the cross amplification of 37 microsatellite markers for their suitability in genotyping the southern anteater-chat Myrmecocichla formicivora, an opportunistic cooperatively breeding passerine bird endemic to southern Africa. Fourteen microsatellite markers were identified as having suitable characteristics, with minor deviation from Hardy-Weinberg equilibrium and little evidence of null alleles. These 14 Primer pairs were combined in 4 multiplexes and run on 183 individual samples from our study population of southern anteater-chat on Benfontein Nature Reserve, near Kimberley in central South Africa. The loci ranged from 3-34 alleles per locus, and observed heterozygosity ranged from 0.45 -0.93. We then tested these 14 microsatellites for their use in examining paternity in a population of southern anteater-chat being studied on Benfontein Nature Reserve, near Kimberley in South Africa. Of the population of 183 individuals (the 2011 population) 93% of the offspring could be allocated a mother, 97% a father, and 87% a parent pair with 95% confidence. The remainder could be allocated at the 80% confidence level. Where mothers could be assigned from observations this was in 100% agreement with the microsatellite results, giving us good support for the accurate assignment of parentage in our population.
Southern anteater-chat, cooperative breeding, social evolution, microsatellites, genotyping, cross-amplification, behavioural ecology, delayed dispersal, family living, Africa
Research subject Biology with specialization in Population Biology
IdentifiersURN: urn:nbn:se:uu:diva-179075OAI: oai:DiVA.org:uu-179075DiVA: diva2:544046