Three-dimensional structure determination with an X-ray laser
(English)Manuscript (preprint) (Other academic)
Three-dimensional structure determination of a non-crystalline virus has been achieved from a set of randomly oriented continuous diffraction patterns captured with an X-ray laser. Intense, ultra-short X-ray pulses intercepted a beam of single mimivirus particles, producing single particle X-ray diffraction patterns that are assembled into a three-dimensional amplitude distribution based on statistical consistency. Phases are directly retrieved from the assembled Fourier distribution to synthesize a three-dimensional image. The resulting electron density reveals a pseudo-icosahedral asymmetric virion structure with a compartmentalized interior, within which the DNA genome occupies only about a fifth of the volume enclosed by the capsid. Additional electron microscopy data indicate the genome has a chromatin-like fiber structure that has not previously been observed in a virus.
Mimivirus, flash diffraction, three dimensional, imaging, CXI
IdentifiersURN: urn:nbn:se:uu:diva-179597OAI: oai:DiVA.org:uu-179597DiVA: diva2:545328
FunderEU, European Research Council