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Electrochemical Synthesis of Gold and Protein Gradients on Particle Surfaces
Uppsala University, Disciplinary Domain of Science and Technology, Physics, Department of Physics and Astronomy, Surface and Interface Science.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Inorganic Chemistry.
2012 (English)In: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 28, no 28, p. 10318-10323Article in journal (Refereed) Published
Abstract [en]

A straightforward, versatile approach to the production of protein gradients on planar and spherical particle surfaces is described. The method is based on the spatially controlled oxidation of thiolated surfaces by Au(III) ions generated via the electrochemical oxidation of a gold electrode in a phosphate-buffered saline solution (10 mM PBS, pH 7.2, 150 mM NaCl). Because the gold electrode is in direct contact with the thiolated surfaces, the released Au(III) ions, which are present as Au(III) chloride complexes, give rise to the formation of a surface gradient of Au(I)-thiolate complexes depending on the local redox potential given by the local Au(III) concentration. As is shown on the basis of the use of X-ray photoelectron spectroscopy and fluorescently labeled proteins, the Au(I)-thiolate complexes can subsequently be functionalized with thiolated proteins, yielding surface density protein gradients on micrometer-sized nonconducting polymer beads as well as linear Au(I)-thiolate gradients on planar silicon surfaces.

Place, publisher, year, edition, pages
2012. Vol. 28, no 28, p. 10318-10323
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-179572DOI: 10.1021/la3017694ISI: 000306441100003OAI: oai:DiVA.org:uu-179572DiVA: diva2:545406
Available from: 2012-08-20 Created: 2012-08-20 Last updated: 2017-12-07Bibliographically approved

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Nyholm, Leif

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