uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Cytochemical evidence for the leakage of acid phosphatase through ultrastructurally intact lysosomal membranes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine.
1972 (English)In: The Histochemical Journal, ISSN 0018-2214, E-ISSN 1573-6865, Vol. 4, no 6, 479-491 p.Article in journal (Refereed) Published
Abstract [en]

Fixation under 'improper' conditions ofin vitro cultivated cells results in an extensive diffusion of the lysosomal enzyme acid phosphatase because of the influence of a low effective osmotic pressure. In the present investigation, advantage was taken of this predictable diffusion in order to establish whether or not leakage of acid phosphatase could take place through ultrastructurally 'intact' lysosomal membranes.In order to reveal small holes in the lysosomal membranes, secondary lysosomes were labelled with thorium dioxide particles, which were presumed to appear free in the cell sap if ruptures in the membranes larger than about Ioo √Ö were created.The experiments revealed that following the fixation ofin vitro cultivated human glia cells under 'improper' conditions, mitochondria and ground cytoplasm show considerable swelling artifacts, while secondary lysosomes appear to be essentially unaffected. The lysosomes, nevertheless, apparently lost most of their content of acid phosphatase, as judged from enzyme cytochemical studies. These findings indicate that leakage of acid phosphatase from ultrastructurally 'intact' lysosomes is possible.

Place, publisher, year, edition, pages
1972. Vol. 4, no 6, 479-491 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-180767DOI: 10.1007/BF01011128ISI: A1972O471500001OAI: oai:DiVA.org:uu-180767DiVA: diva2:551726
Available from: 2012-09-12 Created: 2012-09-10 Last updated: 2017-12-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Faculty of Medicine
In the same journal
The Histochemical Journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 381 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf